Protein_Domain
LbCas12a_p

Part:BBa_K4667000:Design

Designed by: Xuanxin Zhang   Group: iGEM23_BPI-China   (2023-09-27)


Rna-mediated endonuclease can specifically cut target double-stranded DNA in the presence of PAM in


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7911
    Illegal EcoRI site found at 8260
    Illegal XbaI site found at 5030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7911
    Illegal EcoRI site found at 8260
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7911
    Illegal EcoRI site found at 8260
    Illegal BglII site found at 4964
    Illegal BglII site found at 6501
    Illegal BglII site found at 7238
    Illegal XhoI site found at 8818
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7911
    Illegal EcoRI site found at 8260
    Illegal XbaI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7911
    Illegal EcoRI site found at 8260
    Illegal XbaI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
    Illegal AgeI site found at 8152
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 6565
    Illegal SapI.rc site found at 2263


Design Notes

Cas12a requires low-temperature storage


Source

This Cas12a enzyme does not contain any nuclease activity of any kind other than Cas12a. In this product, the NLS nuclear localization signal is incorporated into the C-terminal of AsCas12a, which can be used for intracellular gene editing.

References